Q. No.An enzyme catalysing the removal of nucleotides from ends of DNA is:
1.
DNA ligase
2.
Endonuclease
3.
Exonuclease
4.
Protease
Given below are four statements pertaining to the separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements:
| a. | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| b. | DNA fragments travel along the surface of the gel whose concentration does not affect the movement of DNA. |
| c. | Smaller the size of the DNA fragment, larger the distance it travels through it. |
| d. | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
1. (a), (c) and (d)
2. (a), (b) and (c)
3. (b), (c) and (d)
4. (a), (b) and (d)
A selectable marker is used to:
| 1. | help in eliminating the non-transformants so that the transformants can be regenerated. |
| 2. | identify the gene for the desired trait in an alien organism. |
| 3. | select a suitable vector for transformation in a specific crop. |
| 4. | mark a gene on a chromosome for isolation using a restriction enzyme. |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | iii | i | iv | ii |
| 2. | iii | iv | i | ii |
| 3. | iv | iii | i | ii |
| 4. | ii | iv | i | iii |
Electroporation is:
| 1. | the process of separating charged molecules through a gel maintained in an electric field |
| 2. | the process of combining foreign DNA to an electrically charged vector molecule |
| 3. | the process of introducing DNA into cells by the application of high-voltage pulses |
| 4. | the process of protoplast fusion by the application of high-voltage pulses |
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid
2. Bacteriophages
3. BAC
4. Plasmid
The following palindrome is recognized by the restriction enzyme:
1. BamHI
2. EcoRI
3. HindII
4. PstI
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI
2. HindII
3. SmaI
4. BamHI
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
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