Q. No.The following palindrome is recognized by the restriction enzyme:
1. BamHI
2. EcoRI
3. HindII
4. PstI
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid
2. Bacteriophages
3. BAC
4. Plasmid
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI
2. HindII
3. SmaI
4. BamHI
Electroporation is:
| 1. | the process of separating charged molecules through a gel maintained in an electric field |
| 2. | the process of combining foreign DNA to an electrically charged vector molecule |
| 3. | the process of introducing DNA into cells by the application of high-voltage pulses |
| 4. | the process of protoplast fusion by the application of high-voltage pulses |
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
| 1. | Chilled chloroform | 2. | Isopropanol |
| 3. | Chilled ethanol | 4. | Methanol at room temperature |
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
Restriction endonuclease:
1. cuts the DNA molecule randomly
2. cuts the DNA molecule at specific sites
3. restricts the synthesis of DNA inside the nucleus
4. synthesis DNA
In the screening process, clones that metabolize X-gal turn
1. yellow
2. orange
3. blue
4. colorless
In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with
1. corn
2. rice
3. wheat
4. soy beans
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