Q. No.1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
Given below is a sample of a portion of the DNA strand given the base sequence on the opposite strand. What is so special show in it?
5'- GAATTC...3'
3'- CTTAAG ... 5'
1. Detection mutation
2. Start codon at 5' end
3. Palindromic sequence of base pairs
4. Replication completed
Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
Restriction endonuclease:
1. cuts the DNA molecule randomly
2. cuts the DNA molecule at specific sites
3. restricts the synthesis of DNA inside the nucleus
4. synthesis DNA
A kind of biotechnology involving manipulation of DNA is:
1. DNA replication
2. genetic engineering
3. denaturation
4. renaturation
In recombinant DNA technique, the term vector refers to:
| 1. | Donor DNA, is identified and picked up through electrophoresis |
| 2. | Plasmid transfers DNA into living cell |
| 3. | Collection of the entire genome in the form of plasmid |
| 4. | Enzymes cut the DNA at specific sites |
The vector for T-DNA is:
| 1. | Thermus aquaticus |
| 2. | Salmonella typhimurium |
| 3. | Agrobacterium tumefaciens |
| 4. | Escherichia coli |
Which one is a true statement regarding DNA polymerase used in PCR?
1.
It is used to ligate introduces DNA in recipient cells
2.
It serves as a selectable marker
3.
It is isolated from a virus
4.
It remains active at high temperatures
In the screening process, clones that metabolize X-gal turn
1. yellow
2. orange
3. blue
4. colorless
In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with
1. corn
2. rice
3. wheat
4. soy beans
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