In the first gene cloning experiment:

1.	Researchers successfully identified a human gene responsible for the disease.
2.	Researchers successfully inserted a gene for kanamycin resistance into a plasmid vector.
3.	Researchers demonstrated that many different DNA fragments could insert into a plasmid vector
4.	Researchers produced a strain of bacteriophage with an increased ability to infect E. coli.

An important limitation to the use of Agrobacterium tumefaciens is that it can not:

1. infect dicots

2. be genetically modified

3. be cultured on a nutrient medium

4. infect crop plants such as wheat and corn

Match each item in Column I with one item in Column II and chose your answer from the codes given below:
Column I                             Column II
(restriction enzyme)            (recognition site)
I. EcoRI                              1. GAATTC
II. SmaI                              2. CCCGGG
III. ScaI                              3. AGTACT
IV. BamHI                            4.GGATCC
Codes:
     I    II    III   IV
A.  1    2     3    4
B.  2    1    4    3
C.  2    1    3    4
D.  1    2    4    3

What is true about the steps of PCR shown in the diagram given below?
  

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as:

1. southern blotting
2. northern blotting
3. polymerase chain reaction
4. DNA replication

Agrobacterium tumefaciens is

1. a disease in humans that causes loss of sight
2. a bacterium that can be used to introduce DNA into plants
3. a fungi that is used to produce antibiotics in large amounts
4. a disease in humans that causes loss of weight

Vectors are

1. molecules that degrade nucleic acids
2. molecules that help in replication
3. molecules that are able to covalently bond to and carry foreign DNA into cells
4. molecules that protect host cells from invasion by foreign DNA

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

1. DNA polymerase
2. Restriction endonuclease
3. DNA ligase
4. DNA helicase

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

1. laser

2. DNA probe

3. gene gun

4. inoculating needle

Bacterial resistance to antibiotics is a genetic trait carried in the bacterial:

1. Intron

2. Chromosome

3. Plasmid

4. Centromere