In the following diagram of the lac operon, an operon for inducible enzymes, identify components and enzymes:

 

X

Y

E1

E2

E3

(1)

Repressor protein

Inducer (lactose)

â-Galactosidase

Permease

Transacetylase

(2)

Inducer (lactose)

Repressor protein

â-Galactosidase

Permease

Transacetylase

(3)

Repressor protein

Inducer (lactose)

â-Galactosidase

Transacetylase

Permease

(4)

Repressor protein

Inducer (lactose)

Permease

Transacetylase

â-Galactosidase

Subtopic:  Gene Regulation: Lac Operon |
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Alfred Hershey and Martha Chase made a big contribution in proving DNA role as the hereditary molecule. The experiment is shown in the diagram. A and C are the presence or absence of radioactivity detected in cells B and D are the presence or absence of radioactivity detected in supernatants cells. Identify A, B, C and D:

(a) A - No Radioactivity (35S) detected in cells; B - Radioactivity (32P) detected in supernatant; C - Radioactivity (35S) detected in cells; D - No Radioactivity in supernatant
(b) A- Radioactivity (35S) detected in cells; B - No Radioactivity (35S) detected in supernatant; C - Radioactivity (32P) detected in cells; D - No Radioactivity in supernatant
(c) A - No Radioactivity (35S) detected in cells; B - Radioactivity (35S) detected in supernatant; C - Radioactivity (32P) detected in cells; D - No Radioactivity in supernatant
(d) A - No Radioactivity (35S) detected in cells; B - Radioactivity (35S) detected in supernatant; C - No Radioactivity (32P) detected in cells; D - Radioactivity in supernatant

Subtopic:  Search for Genetic Material: II | Search for Genetic Material |
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If the DNA of this virus was labeled with 32P and the protein of the virus was labeled with 35S, which molecule(s) would be present inside the cells?
(1) 35S and 32P

(2) 35S only

(3) 32P only

(4) Neither molecule would be present inside the cell

Subtopic:  Search for Genetic Material: II | Search for Genetic Material |
 81%
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Which of the following statements is correct?
I. The biochemical nature of genetic material was not defined from the experiments conducted by Griffith
II. Working on transformation Avery et al concluded DNA is genetic material but not all biologists were convinced
III. RNA is the genetic material in TMV, QB bacteriophage etc.
IV. DNA is the predominant genetic material while RNA performs dynamic functions of messenger adapter
V. Viruses having DNA genome and having shorter life span mutate and evolve faster
(1) All are correct             

(2) I and II             

(3) All except V               

(4) III and IV

Subtopic:  Search for Genetic Material:I | Search for Genetic Material: II | DNA vs RNA as Genetic Material | Search for Genetic Material |
 66%
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The prediction of sequence of amino acid is _____A ____ if the sequence of nucleotides mRNA is known. The prediction of sequence of nucleotide is concerned mRNA is ____B____ because the genetic code is _____C_____:
(1) A - possible, B - impossible, C - universal

(2) A - possible, B - impossible, C - degenerate

(3) A - impossible, B - possible, C - universal

(4) A - impossible, B - possible, C - degenerate

Subtopic:  Genetic Code: III | Genetic Code |
 62%
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Choose the correct statements:
I. Binding of mRNA to the ribosome is loose and reversible.
II. The ribosome, which is composed of tRNA and proteins, consists of a small and large subunit.
III. Each of the 20 amino acids has a minimum of one specific tRNA molecule.
IV. tRNAMET binds to the first codon (AUG) - this is called the START codon.
V. The second tRNA binds to the second codon so that their attached monosaccharides are next to each other
VI. The newly formed polypeptide may undergo post-transcriptional modification.
(1) I, III and IV         

(2) II, IV, VI               

(3) II, V and VI           

(4) I, V, VI

Subtopic:  Translation Mechanism |
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The length of DNA has 45000 base pairs. How many complete turns will the DNA molecule take?

(1) 45000            

(2) 450                

(3) 4500               

(4) 45

Subtopic:  DNA Double Helix |
 87%
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Go through the following facts:
I. 'AAG' always code for lysine. It cannot code for any other amino acid.
II. Serine amino acid can be coded by UCU / UCC / UCA or UCG.
III. UUU form phenylalanine in bacteria as well as human beings.
IV. UUU GGG CCC AAA Me codes for five amino acids.
Now point out nature of genetic code after reading above:
(1) 1 – Specificity / Non-ambiguity of code; II - Universality of code; III - Degeneracy of code; IV - Triplet and non-overlapping code

(2) I – Specificity / Non-ambiguity of code; II - Degeneracy of code; III - Universality of code; IV - Triplet and non-overlapping code

(3) 1 – Specificity / Non-ambiguity of code; II - Degeneracy of code; III - Universality of code; IV - Triplet and overlapping code

(4) 1 – Specificity / Non-ambiguity of code; II - Universality of code; III - Degeneracy of code; IV - Triplet and overlapping code

Subtopic:  Genetic Code: III | Genetic Code |
 73%
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The full form of ELSI is:

(1) Embedded Low Software Index    

(2) Ear Lung Spleen Immunity

(3) Ethical Legal and Social Issue      

(4) Endonuclease Ligase Surface Immunity

Subtopic:  Human Genome Project |
 90%
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DNA fingerprinting works because:
(1) Genes containing the same alleles make it simple to compare different individuals

(2) PCR allows amplification of proteins from single cells

(3) There are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual

(4) DNA in the skin cells is very diverse

Subtopic:  DNA Fingerprinting |
 69%
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