Select Question Set:
Q. No.Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
Column-I
Column-II
(a)
Heat denaturation
(i)
70-75°C
(b)
Annealing
(ii)
90-98°C
(c)
Primer extension
(iii)
40-60°C
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
66%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Consider the given two statements:
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: Tools |
73%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Subtopic: Process of Biotech |
91%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Which of the following does not hold true for restriction enzymes?
| 1. | It recognises a palindromic nucleotide sequence. |
| 2. | It is an endonuclease. |
| 3. | It is isolated from viruses. |
| 4. | it can produce the same kind of sticky ends in different DNA molecules. |
Subtopic: Restriction Enzymes - Main Enzymes |
81%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
| Assertion (A): | Bacterium protects its own DNA by changing DNA sequence. |
| Reason (R): | Methylation of DNA nucleotides is carried out by modification enzymes. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 4: Below 35%
Please attempt this question first.
Hints
Please attempt this question first.
Name the enzymes 'P' and 'Q' that are involved in the processes given below.
| 1. | Enzyme P-Exonuclease and Enzyme Q-permease. |
| 2. | Enzyme P-Exonuclease and Enzyme Q-Ligase. |
| 3. | Enzyme P-Endonuclease and Enzyme Q-permease. |
| 4. | Enzyme P-Restriction endonuclease and Enzyme Q-Ligase. |
Subtopic: Restriction Enzymes - Main Enzymes |
80%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
| Assertion (A): | During the heat shock method, the cells are incubated with recombinant DNA at \(42^{\circ} \mathrm{C}\), following by placing them briefly on ice and then putting if back at \(42^{\circ} \mathrm{C}\). |
| Reason (R): | After heat shock, uptake of DNA through transient pores in the bacterial cell wall takes place. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Transforming Plant & Animal Cell |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
In the diagram given below A, B, and C are:
1. A: Bam HI, B: ampR, C: Pst I
2. A: Bam HI, B: Pst I, C: ampR
3. A: Pst I, B: Bam, C: ampR
4. A: ampR, B: Pst I, C: Bam HI
1. A: Bam HI, B: ampR, C: Pst I2. A: Bam HI, B: Pst I, C: ampR
3. A: Pst I, B: Bam, C: ampR
4. A: ampR, B: Pst I, C: Bam HI
Subtopic: Cloning Vector |
86%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Rajesh was doing gel electrophoresis to purify DNA fragments. Given diagram is the sketch of the observations of the experiment performed by him.
At which end he would have loaded the samples?
1. He would have loaded the samples near end A.
2. He would have loaded the samples near end B.
3. He would have loaded the samples at centre of AB.
4. He would have loaded the samples anywhere between A and B.
At which end he would have loaded the samples?
1. He would have loaded the samples near end A.
2. He would have loaded the samples near end B.
3. He would have loaded the samples at centre of AB.
4. He would have loaded the samples anywhere between A and B.
Subtopic: Separation and Isolation of DNA fragments |
84%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
The role of DNA ligase in the construction of a recombinant DNA molecule is:
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
Subtopic: Tools: Enzymes: II |
Level 4: Below 35%
Please attempt this question first.
Hints
Please attempt this question first.
Select Question Set:
© 2026 GoodEd Technologies Pvt. Ltd.