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Q. No.Which of the following does not hold true for restriction enzymes?
1.
It recognises a palindromic nucleotide sequence.
2.
It is an endonuclease.
3.
It is isolated from viruses.
4.
it can produce the same kind of sticky ends in different DNA molecules.
Subtopic: Restriction Enzymes - Main Enzymes |
81%
Level 1: 80%+
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| Assertion (A): | Bacterium protects its own DNA by changing DNA sequence. |
| Reason (R): | Methylation of DNA nucleotides is carried out by modification enzymes. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 4: Below 35%
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Name the enzymes 'P' and 'Q' that are involved in the processes given below.
| 1. | Enzyme P-Exonuclease and Enzyme Q-permease. |
| 2. | Enzyme P-Exonuclease and Enzyme Q-Ligase. |
| 3. | Enzyme P-Endonuclease and Enzyme Q-permease. |
| 4. | Enzyme P-Restriction endonuclease and Enzyme Q-Ligase. |
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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| Assertion (A): | During the heat shock method, the cells are incubated with recombinant DNA at \(42^{\circ} \mathrm{C}\), following by placing them briefly on ice and then putting if back at \(42^{\circ} \mathrm{C}\). |
| Reason (R): | After heat shock, uptake of DNA through transient pores in the bacterial cell wall takes place. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Transforming Plant & Animal Cell |
Level 3: 35%-60%
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In the diagram given below A, B, and C are:
1. A: Bam HI, B: ampR, C: Pst I
2. A: Bam HI, B: Pst I, C: ampR
3. A: Pst I, B: Bam, C: ampR
4. A: ampR, B: Pst I, C: Bam HI
1. A: Bam HI, B: ampR, C: Pst I2. A: Bam HI, B: Pst I, C: ampR
3. A: Pst I, B: Bam, C: ampR
4. A: ampR, B: Pst I, C: Bam HI
Subtopic: Cloning Vector |
86%
Level 1: 80%+
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Rajesh was doing gel electrophoresis to purify DNA fragments. Given diagram is the sketch of the observations of the experiment performed by him.
At which end he would have loaded the samples?
1. He would have loaded the samples near end A.
2. He would have loaded the samples near end B.
3. He would have loaded the samples at centre of AB.
4. He would have loaded the samples anywhere between A and B.
At which end he would have loaded the samples?
1. He would have loaded the samples near end A.
2. He would have loaded the samples near end B.
3. He would have loaded the samples at centre of AB.
4. He would have loaded the samples anywhere between A and B.
Subtopic: Separation and Isolation of DNA fragments |
84%
Level 1: 80%+
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The role of DNA ligase in the construction of a recombinant DNA molecule is:
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
Subtopic: Tools: Enzymes: II |
Level 4: Below 35%
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In the processes of Recombinant DNA Technology, downstream processing:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | occurs after the completion of the biosynthetic stage. |
| II: | involves processes that make products ready for marketing as a finished product. |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Bioreactors/Downstream Processing |
92%
Level 1: 80%+
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
90%
Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | In most recombinant technologies, there is a need for the recombinant DNA to be expressed. |
| Reason (R): | In most recombinant technologies, the ultimate aim is to produce a desirable protein. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Obtaining Copy of Gene from Donor DNA |
77%
Level 2: 60%+
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