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Q. No.Rajesh was doing gel electrophoresis to purify DNA fragments. Given diagram is the sketch of the observations of the experiment performed by him.
At which end he would have loaded the samples?
1. He would have loaded the samples near end A.
2. He would have loaded the samples near end B.
3. He would have loaded the samples at centre of AB.
4. He would have loaded the samples anywhere between A and B.
Subtopic: Separation and Isolation of DNA fragments |
Level 4: Below 35%
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The role of DNA ligase in the construction of a recombinant DNA molecule is:
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
1. Formation of phosphodiester bond between two DNA fragments.
2. Formation of hydrogen bond between sticky end of DNA fragments.
3. Ligation of all purine and pyrimidine bases.
4. None of the above.
Subtopic: Tools: Enzymes: II |
Level 4: Below 35%
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In the processes of Recombinant DNA Technology, downstream processing:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | occurs after the completion of the biosynthetic stage. |
| II: | involves processes that make products ready for marketing as a finished product. |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Bioreactors/Downstream Processing |
92%
Level 1: 80%+
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
90%
Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | In most recombinant technologies, there is a need for the recombinant DNA to be expressed. |
| Reason (R): | In most recombinant technologies, the ultimate aim is to produce a desirable protein. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Obtaining Copy of Gene from Donor DNA |
77%
Level 2: 60%+
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Consider the given two statements:
| Assertion (A): | The most commonly used bioreactors for biosynthesis are of stirring type. |
| Reason (R): | A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is False |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Bioreactors/Downstream Processing |
56%
Level 3: 35%-60%
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After being carried about for 30 cycles, PCR can amplify a single segment of DNA to approximately:
| 1. | 1000 copies | 2. | One lakh copies |
| 3. | One million copies | 4. | One billion copies |
Subtopic: Polymerase Chain Reaction: PCR |
87%
Level 1: 80%+
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The source of thermostable DNA polymerase used in Polymerase Chain Reaction is:
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
1. a blue green alga.
2. a bacterium.
3. a yeast.
4. a protozoan.
Subtopic: Polymerase Chain Reaction: PCR |
92%
Level 1: 80%+
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
81%
Level 1: 80%+
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While using bacterial cell as a source, which process of Recombinant DNA Technology will need lysozyme?
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
1. Isolation of the Genetic Material
2. Cutting of DNA at Specific Locations
3. Amplification of Gene of Interest
4. Insertion of Recombinant DNA into the Host Cell
Subtopic: Process of Biotech |
78%
Level 2: 60%+
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