Which of the following is a endonuclease?
(1) Protease
(2) DNase I
(3) RNase restriction
(4) Hind II
The Taq polymerase enzyme is obtained from
1. Thiobacillus ferroxidans
2. Bacillus subtilis
3. Pseudomonas subtilis
4. Thermus aquaticus
Golden rice is a genetically modified crop plant where the incorporated gene is meant for
biosynthesis of
1. vitamin-B
2. omega 3
3. vitamin-C
4. vitamin-A
The cutting of DNA at specific locations became possible with the discovery of
(1) restriction enzymes
(2) probes
(3) selectable markets
(4) ligases
The DNA molecule to which the gene of interest is integrated for cloning is called:
1. | Transformer | 2. | Vector |
3. | Template | 4. | Carrier |
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
(1) centrifugation
(2) polymerase chain reaction
(3) electrophoresis
(4) restriction mapping
Which of the following is not correctly matched for the organism and its cell wall
degrading enzyme?
1. Bacteria —Lysozyme
2. Plant cells — Cellulase
3. Algae —Methylase
4. Fungi — Chitinase
The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, Eco RI -selectable markers
4. ampR, tetR-antibiotic resistance genes
PCR and Restriction Fragment Length Polymorphism are the methods for
1. study of enzymes
2. genetic transformation
3. DNA sequencing
4. genetic fingerprinting