Hind II

1.      First restriction endonuclease

2.      Was characterized 7 years later

3.      Specific sequence of 5 base pairs

4.     All of these

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1.      700; 350

2.      900; 230

3.      600; 270

4.     550; 210

Which of the following is correct representation of the name of enzyme?

1.      ECORI

2.      EcoRI

3.      ECoRI

4.     ECoR1

EcoRI comes from

1.      Escherichia coli RY13

2.      Escherichia coli RY16

3.      Escherichia coli RY19

4.     Escherichia coli RY12

In EcoRI, here I stands for

1.      Order in which strains are discovered

2.      Order in which enzymes are isolated

3.      Order in which enzymes are isolated from that strain

4.      Order in which strains are reported in microbiology

Restriction endonuclease

1.      Directly recognizes a specific sequence

2.      Makes the cut at the sugar-phosphate core.

3.      Comes under nuclease

4.      Both 1 and 3

Restriction endonuclease cuts

1. At palindromic sequences for some enzymes not for all

2. After scanning and recognition of specific sequence in a DNA strand

3. Cuts the strand exactly at the centre

4. All of these

Restriction enzyme EcoRI cuts

1.      Between bases G and A

2.      Cuts between GAATTC

3.      Cuts between G and A  at GAATTC

4.      Both 1 and 3

Restriction endonuclease cuts

1.      Exactly at the center of site

2.      Cutting of both the strands occur one after another

3.      Between the same two bases on the opposite strands

4.     Both 1 and 3

EcoRI cuts

1.      A little away from the centre

2.      Both the strands at the same time

3.      Creates blunt ends

4.      Both 1 and 2