Restriction endonuclease

1.      Directly recognizes a specific sequence

2.      Makes the cut at the sugar-phosphate core.

3.      Comes under nuclease

4.      Both 1 and 3

Restriction endonuclease cuts

1. At palindromic sequences for some enzymes not for all

2. After scanning and recognition of specific sequence in a DNA strand

3. Cuts the strand exactly at the centre

4. All of these

Restriction enzyme EcoRI cuts

1.      Between bases G and A

2.      Cuts between GAATTC

3.      Cuts between G and A  at GAATTC

4.      Both 1 and 3

Restriction endonuclease cuts

1.      Exactly at the center of site

2.      Cutting of both the strands occur one after another

3.      Between the same two bases on the opposite strands

4.     Both 1 and 3

EcoRI cuts

1.      A little away from the centre

2.      Both the strands at the same time

3.      Creates blunt ends

4.      Both 1 and 2

DNA gel electrophoresis is commonly prepared by

1.      Agarose, a natural polymer

2.      Extracted from seaweeds

3.      A synthetic polymer

4.      Both 1 and 2

Which of the following step is first one in order to separate DNA fragments?

1.      Running of gel

2.      Staining of gel

3.      Elution

4.     Setting of gel

Which of the following step is last for isolating the desired DNA fragment?

1.      Running of gel

2.      Elution

3.      Staining

4.     Visualization

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning