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Q. No.Match the restriction enzyme given in Column-I with their source given in Column-II and choose the correct option.
Column-I
Column-II
i.
Alu I
a.
Bacillus amyloliquefaciens H
ii.
Bam HI
b.
H. influenza RD
iii.
Eco RI
c.
Arthobacter luteus
iv.
Hind II
d.
Escherichia coli Ry 13
1. i-a, ii-c, iii-d, iv-b
2. i-c, ii-b, iii-d, iv-a
3. i-c, ii-d, iii-a, iv-b
4. i-c, ii-a, iii-d, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
76%
Level 2: 60%+
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Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and Select the correct option.
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
| Column I (Restriction Enzyme) |
Column II (Recognition sequence) |
||
| i. | AluI | P. | \(5'---G- \downarrow-A-T-C-C---3'\\3'---C-C-T-A-G-\uparrow-G---5'\) |
| ii, | BamHI | Q. | \(5'---A-G-\downarrow-C-T---3'\\ 3' ---T-C- \uparrow -G-A---5' \) |
| iii. | EcoRI | R. | \(5'--G-T-C- \downarrow - G-A-C--3' \\ 3'--C-A-G-\uparrow-C-T-G--5'\) |
| iv. | HindII | S. | \(5'---G- \downarrow-A-A-T-T-C---3'\\ 3'--- C-T-T-A-A-\uparrow-G---5'\) |
1. (i)-(R),(ii)-(P), (iii)-(S), (iv)-(Q)
2. (i)-(S),(ii)-(P), (iii)-(Q), (iv)-(R)
3. (i)-(P),(ii)-(Q), (iii)-(S), (iv)-(R)
4. (i)-(Q),(ii)-(P), (iii)-(S), (iv)-(R)
Subtopic: Tools: Vectors: I |
Level 3: 35%-60%
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Match Column-I with column II and select the correct option
1. (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | DNA fragmentation | (i) | ssDNA gets embedded in nylon membrane |
| (b) | Electrophoresis | (ii) | Sample DNA and probe DNA from double stranded DNA |
| (c) | Southern blotting | (iii) | DNA fragmentation move to positive pole |
| (d) | Hybridization | (iv) | Fragments of DNA with variable length are formed |
2. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
3. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Tools |
82%
Level 1: 80%+
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Match Column-I with column II and select the correct option
1. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophosis | (i) | To combine genes from two organisms |
| (b) | cDNA | (ii) | To make a copy of DNA from mRNA |
| (c) | Genetic Engineering | (iii) | Separate the DNA fragments |
| (d) | PCR | (iv) | Gene multiplication in vitro |
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
4. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
Subtopic: Tools |
80%
Level 1: 80%+
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Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | 70-75°C |
| (b) | Annealing | (ii) | 90-98°C |
| (c) | Primer extension | (iii) | 40-60°C |
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
64%
Level 2: 60%+
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Identify the process in Column-I with the term in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
| Column-I | Column-II | ||
| (a) | Amplifications of gene of interest | (i) | Downstream processing |
| (b) | Large scale production of plants in short duration | (ii) | Polymerase chain reaction |
| (c) | Separation and purification of desired product | (iii) | Transformation |
| (d) | Insertion of rDNA into host | (iv) | Micro propagation |
2. (a)-(ii), (b)-(iv), (c)-(i), (d)-(iii)
3. (a)-(ii), (b)-(iv), (c)-(iii), (d)-(i)
4. (a)-(iv), (b)-(ii), (c)-(i), (d)-(iii)
Subtopic: Bioreactors/Downstream Processing |
72%
Level 2: 60%+
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Match the restriction enzyme given in Column-I with their source given in column II and choose the correct option
1. (a)-(i), (b)-(iii), (c)-(iv), (d)-(ii)
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
| Column-I | Column-II | ||
| (a) | Alu I | (i) | Bacillus amyloliquefaciens H |
| (b) | Bam HI | (ii) | H. influenza Rd. |
| (c) | Eco RI | (iii) | Arthobacter luteus |
| (d) | Hind RI | (iv) | Escherichia coli Ry 13 |
2. (a)-(iii), (b)-(i), (c)-(iv), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
Subtopic: Tools: Vectors: I |
85%
Level 1: 80%+
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Match the steps of PCR in Column-I with the process in column II and select the correct option
1. (a)-(iii), (b)-(ii), (c)-(i)
2. (a)-(ii), (b)-(iii), (c)-(i)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(i), (c)-(iii)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | addition of nucleotides on template |
| (b) | Annealing | (ii) | separation of two strands of DNA |
| (c) | Primer Extension | (iii) | paring of primer to complementary sequence in DNA |
2. (a)-(ii), (b)-(iii), (c)-(i)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(i), (c)-(iii)
Subtopic: Polymerase Chain Reaction: PCR |
65%
Level 2: 60%+
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Match the technique in Column-I with the terms given in column II
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophoresis | (i) | Chrimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Process of Biotech |
79%
Level 2: 60%+
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Consider the given two statements:
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: Tools |
74%
Level 2: 60%+
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