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Q. No.Match the steps of PCR in Column-I with the process in column II and select the correct option
Column-I
Column-II
(a)
Heat denaturation
(i)
addition of nucleotides on template
(b)
Annealing
(ii)
separation of two strands of DNA
(c)
Primer Extension
(iii)
paring of primer to complementary sequence in DNA
1. (a)-(iii), (b)-(ii), (c)-(i)
2. (a)-(ii), (b)-(iii), (c)-(i)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(i), (c)-(iii)
Subtopic: Polymerase Chain Reaction: PCR |
55%
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Match the technique in Column-I with the terms given in column II
1. (a)-(iv), (b)-(ii), (c)-(iii), (d)-(i)
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
| Column-I | Column-II | ||
| (a) | Gel electrophoresis | (i) | Chrimeric DNA |
| (b) | Restriction endonuclease | (ii) | In vitro DNA multiplication |
| (c) | rDNA technology | (iii) | Palindrome |
| (d) | PCR | (iv) | Separation of DNA fragments |
2. (a)-(iv), (b)-(i), (c)-(iii), (d)-(ii)
3. (a)-(iii), (b)-(iv), (c)-(i), (d)-(ii)
4. (a)-(iv), (b)-(iii), (c)-(i), (d)-(ii)
Subtopic: Process of Biotech |
73%
From NCERT
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Consider the given two statements:
1. Both Assertion and Reason are true but Reason does not correctly explain the Assertion.
2. Assertion is false but Reason is true.
3. Assertion is true but Reason is false.
4. Both Assertion and Reason are true and Reason correctly explains the Assertion.
| Assertion: | A piece of DNA if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason: | The vectors provide the origin of replication. |
2. Assertion is false but Reason is true.
3. Assertion is true but Reason is false.
4. Both Assertion and Reason are true and Reason correctly explains the Assertion.
Subtopic: Tools |
82%
From NCERT
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Introduction of an alien DNA into plant host cell is achieved by making them
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
Subtopic: Transforming Plant & Animal Cell |
61%
From NCERT
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Subtopic: Process of Biotech |
93%
From NCERT
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Which of the following does not hold true for restriction enzymes?
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
1. It recognises a palindromic nucleotide sequence.
2. It is an endonuclease.
3. It is isolated from viruses.
4. it can produce the same kind of sticky ends in different DNA molecules.
Subtopic: Restriction Enzymes - Main Enzymes |
81%
From NCERT
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Name the enzymes 'P' and 'Q' that are involved in the processes given below.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
Subtopic: Restriction Enzymes - Main Enzymes |
80%
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| Assertion (A): | During the heat shock method, the cells are incubated with recombinant DNA at \(42^{\circ} \mathrm{C}\), following by placing them briefly on ice and then putting if back at \(42^{\circ} \mathrm{C}\). |
| Reason (R): | After heat shock, uptake of DNA through transient pores in the bacterial cell wall takes place. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Transforming Plant & Animal Cell |
From NCERT
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Name the British biochemist who received the Nobel Prize in Chemistry twice – once for determining the amino acid sequence of insulin and then for developing first-ever DNA sequencing technique:
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
1. Karl Barry Sharpless
2. John Bardeen
3. Frederick Sanger
4. Walter Gilbert
Subtopic: Introduction & History |
84%
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
92%
From NCERT
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