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Q. No.During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic: General Design of an rDNA experiment |
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Level 1: 80%+
NEET - 2013
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Assertion (A): Inserting alien DNA in pBR322 plasmid at Pst I makes the transformants sensitive to ampicillin antibiotic.
Reason (R): Restrictions site for Pst I is present on ampR .
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): Restrictions site for Pst I is present on ampR .
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Vectors: I | Tools |
84%
Level 1: 80%+
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Assertion (A): DNA ligase plays important role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: General Design of an rDNA experiment |
90%
Level 1: 80%+
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Assertion (A): Restriction enzymes belong to a larger class of enzymes called nucleases.
Reason (R): Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the DNA
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): Each restriction enzyme recognizes a specific palindromic nucleotide sequence in the DNA
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Restriction Enzymes - Main Enzymes |
70%
Level 2: 60%+
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Assertion (A): An alien DNA is linked with the origin of replication for making multiple identical copies.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: General Design of an rDNA experiment |
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Level 1: 80%+
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If you want to isolate DNA from a fungal cell, the enzyme that should not be used for the purpose is:
1. Protease
2. RNase
3. DNase
4. Chitinase
1. Protease
2. RNase
3. DNase
4. Chitinase
Subtopic: Process of Biotech |
57%
Level 3: 35%-60%
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If one wants to recover many copies of the target DNA, it should be cloned in a vector:
| 1. | that is a single-stranded DNA molecule |
| 2. | whose origin [ori] supports a high copy number |
| 3. | that carries two selectable markers |
| 4. | whose cloning sites are all clustered at one place |
Subtopic: Cloning Vector |
92%
Level 1: 80%+
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In agarose gel electrophoresis, the DNA fragments separate (resolve) according to their:
1. charge
2. size
3. 3-D shape
4. base composition
1. charge
2. size
3. 3-D shape
4. base composition
Subtopic: Separation and Isolation of DNA fragments |
93%
Level 1: 80%+
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The enzyme used in PCR:
| I: | is a DNA-dependent DNA polymerase |
| II: | extends the primers in template independent manner |
| III: | is thermostable |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
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Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism comes under:
1. Bioprocess engineering
2. Genetic engineering
3. Bioinformatics
4. Biomedical technology
1. Bioprocess engineering
2. Genetic engineering
3. Bioinformatics
4. Biomedical technology
Subtopic: General Design of an rDNA experiment |
90%
Level 1: 80%+
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