Q. No.In agarose gel electrophoresis, the DNA fragments separate (resolve) according to their:
1. charge
2. size
3. 3-D shape
4. base composition
I: is a DNA-dependent DNA polymerase
II: extends the primers in template independent manner
III: is thermostable
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
1. Bioprocess engineering
2. Genetic engineering
3. Bioinformatics
4. Biomedical technology
1. Origin of replication (Ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Being linear rather than circular
1. It is extrachromosomal DNA
2. It is circular and double stranded
3. It gets replicated with the help of ori of chromosomal DNA
4. It confers characters like resistance to antibiotics to bacterial cells
Restriction enzymes produced by some bacteria:
1. cleave phosphodiester bond in the main chromosomal DNA of the bacterium.
2. cut single stranded DNA at random positions.
3. prevent the multiplication of bacteriophage in bacteria.
4. provide resistance against antibiotics.
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
The DNA fragments separated on agar gel by electrophoresis can be visualised:
1. without staining in the visible light
2. without staining on exposure to UV light
3. after staining with polyethylene glycol followed by exposure to IR radiation
4. after staining with ethidium bromide followed by exposure to UV radiation
1. PstI
2. PvuII
3. BamH I
4. EcoR I
If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
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