Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:

1.	The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
2.	The enzyme cuts DNA molecules at an identified position within the DNA
3.	The enzyme binds DNA at specific sites and cuts only one of the two strands
4.	The enzyme cuts the sugar-phosphate backbone at specific sites on each strand

DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:

Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?

Biolistic technique is used in:

1. Gene transfer process

2. Tissue culture process

3. Hybridization process

4. Germplasm conservation process

The thermostable enzymes, Taq and Pfu, Isolated from thermophilic bacteria are:

1. RNA polymerases

2. DNA ligases

3. DNA polymerases

4. Restriction endonucleases

The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI

2. HindII

3. SmaI

4. BamHI

The following palindrome is recognized by the restriction enzyme:

1. BamHI

2. EcoRI

3. HindII

4. PstI

Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid

2. Bacteriophages

3. BAC

4. Plasmid

A kind of biotechnology involving manipulation of DNA is:

1. DNA replication

2. genetic engineering

3. denaturation

4. renaturation

Given below is a sample of a portion of the DNA strand given the base sequence on the opposite strand. What is so special show in it? 
5'- GAATTC...3' 
3'- CTTAAG ... 5'

1. Detection mutation

2. Start codon at 5' end

3. Palindromic sequence of base pairs

4. Replication completed