Q. No.A piece of nucleic acid used to find a gene, by forming a hybrid with it, is called a
1. Probe
2. Vector
3. Restriction sequence
4. Retrovirus
1. Probe
2. Vector
3. Restriction sequence
4. Retrovirus
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In situ hybridization is used to :
| 1. | Remove mutations from a chromosome |
| 2. | Remove plasmids from a cell |
| 3. | Clone a gene |
| 4. | Locate a gene on a chromosome |
A bacterium adds methyl group to its DNA, by a process known as modification, in order to:
| 1. | Clone its DNA |
| 2. | Turn its genes on |
| 3. | Transcribe many genes simultaneously |
| 4. | Protect DNA from its own restriction enzymes |
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A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have
1. Been fragmented by the same restriction enzyme
2. Sticky ends
3. Plasmid component
4. Attached methyl group
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Hybridization of mRNA with DNA can reveal which segment of the DNA are introns and which segment are exons. When the magnification is high enough to see the molecule, the introns appear as
1. Single stranded loops in a double stranded molecule
2. Separate fragments
3. Triple stranded nucleic acid molecules
4. Barr bodies
Which of the following primers would allow copying of the single-stranded DNA sequence 5' ATGCCTAGGTC?
| 1. | 5' TACGG | 2. | 5' CTGGA |
| 3. | 5' GACCT | 4. | 5' GGCAT |
DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments are cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is referred to as :
| 1. | restriction map | 2. | RFLP profile |
| 3. | F' factor | 4. | library |
Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
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A key feature of insertional mutagenesis for the identification of plasmids containing recombinant DNA is:
| 1. | the production of nutritional auxotrophs. |
| 2. | the DNA sequencing of recombinant plasmids. |
| 3. | the production of restriction endonuclease maps of recombinant plasmids. |
| 4. | the disruption of a gene on the plasmid by the inserted recombinant DNA. |
Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell?
1. electroporation
2. liposome transfer
3. microinjection
4. particle bombardment
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