What is the final step of PCR?
1. Extension
2. Annealing
3. Denaturation
4. Extraction
After 30 cycles of PCR, how many times DNA will be amplified?
1. Thousand times
2. Million times
3. Billion times
4. Hundreds times
What does continuous culture mean?
1. Where DNA and protein getting expressed continuously
2. Where used medium is drained out and the fresh medium is added from another side
3. Where cells are producing protein for one week continuously
4. Where the production of recombinant DNA is continuing without interference.
Use of primers, occurs in which step of PCR?
1. | Annealing |
2. | Extension |
3. | Denaturation |
4. | Both extension and Annealing |
The volume of bioreactors cannot be
1. | 20 L | 2. | 100L |
3. | 150L | 4. | 200L |
Continuous culture leads to
1. Smaller biomass
2. Higher yields of desired proteins
3. Both 1 and 2
4. Higher yields of recombinant DNA
Bioreactors don't provide which of the conditions?
1. Salts
2. Vitamins
3. Cells
4. Oxygen
The ultimate aim of maximum recombinant DNA technologies is
1. To multiply DNA
2. To express DNA
3. To multiply mRNA
4. To express protein
Continuous culture has
1. Cells in their physically active phase
2. Lag phase
3. Both A and B
4. Exponential phase
Which of the following processes is not associated with the protein produced by recombinant DNA?
1. Restriction Digestion
2. Expression
3. Optimization
4. Purification