Which of the following step is first one in order to separate DNA fragments?

1.      Running of gel

2.      Staining of gel

3.      Elution

4.     Setting of gel

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

Which of the following step is last for isolating the desired DNA fragment?

1.      Running of gel

2.      Elution

3.      Staining

4.     Visualization

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 81%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Subtopic:  Tools: Enzymes: I | Tools |
 58%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

Which of the following steps will be performed after restriction digestion?

1.      Ligation

2.      Elution from DNA gel

3.      Transformation

4.      Cloning

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 71%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

1. a- bacteriophage

2. a- Plasmid

3. a- bacteriophage or Plasmid

4. a- prion

Subtopic:  Tools: Vectors: II | Tools |
 64%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

Which of the following is not a feature to facilitate cloning into a vector?

1.      Origin of replication

2.      Selectable Marker

3.      Multiple cloning site

4.      Virulence region

Subtopic:  Tools: Vectors: I | Tools: Vectors: II | Tools |
 70%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

If I want to check the expression of my gene, then I should use

1.      Vectors supporting high copy number

2.      Vectors supporting low copy number

3.      No need to bother about copy number

4.     Vectors with strong Ori

Subtopic:  Tools: Vectors: II | Tools |
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

Selectable marker helps

1.      To identify transformants

2.      To identify recombinants

3.      To identify non- recombinants

4.      To eliminate transformants

Subtopic:  Selectable Markers: I | Selectable Markers: II | Selectable Markers |
 55%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

The normal E. coli cells have

1.      Kanamycin

2.      Tetracycline

3.      Ampicillin

4.     No resistance

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
 86%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh

For easiest cloning, one needs to get vector with

1.      Single cloning site

2.      Multiple cloning site

3.      Few cloning site

4.     No cloning site at all

Subtopic:  Tools: Vectors: I | Tools |
 68%
From NCERT
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2021 - Achiever Batch - Aryan Raj Singh