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Q. No.Insertional inactivation of the lac Z gene forms-
1.
Blue recombinant colonies
2.
Colourless recombinant colonies
3.
Fluorescent green colonies
4.
There is no relation between the lac Z gene and colour of the colony.
| 1. | Blue recombinant colonies |
| 2. | Colourless recombinant colonies |
| 3. | Fluorescent green colonies |
| 4. | There is no relation between the lac Z gene and colour of the colony. |
Subtopic: Selectable Markers: III | Selectable Markers |
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A reporter enzyme used in biotechnology is-
| 1. | Green Fluorescent Protein |
| 2. | Luciferase |
| 3. | b Galactosidase |
| 4. | All of the above |
Subtopic: Selectable Markers: III | Selectable Markers |
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Downstream processing does not include
(1) Quality control
(2) Clinical trials
(3) Product purification
(4) Sampling from bioreactor
Subtopic: Large Scale Production: II | Large Scale Production |
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Which is not a component of the vector pBR322?
| 1. | Chloramphenicol selectable marker |
| 2. | Replication proteins |
| 3. | Origin of replication |
| 4. | Cloning site for BamHI enzyme |
Subtopic: Tools: Vectors: II | Tools |
75%
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Subtopic: Tools: Vectors: II | Tools |
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Two bacteria found to be very useful in genetic engineering are
(1) Diplococcus and Streptomyces
(2) Rhizobium and Xanthomonas
(3) Vibrio and Escherischia
(4) Escherischia and Agrobacterium
Subtopic: Tools: Vectors: II | Host & Desired DNA: I | Host & Desired DNA | Tools |
97%
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Which of the following would you choose as the safest and least cumbersome selectable marker-
| 1. | Ampicillin resistance gene |
| 2. | Tetracyclin resistance gene |
| 3. | Kanamycin resistance gene |
| 4. | b Galactosidase gene |
Subtopic: Selectable Markers: III | Selectable Markers |
69%
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Recombinant DNA is formed in nature during-
| 1. | Crossing over at pachytene |
| 2. | Integration of plasmid to form episome |
| 3. | Integration of phage DNA to form prophage |
| 4. | More than one is correct. |
Subtopic: Tools: Vectors: I | Tools |
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Southern Blotting essentially makes use of a
| 1. | Buffer | 2. | Electrodes |
| 3. | Agarose gel | 4. | All of the above |
Subtopic: Process of Biotech |
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The native charge on DNA molecules is
| 1. | Positive | 2. | Negative |
| 3. | Neutral | 4. | DNA is an ampholyte |
Subtopic: Process of Biotech |
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