To be useful in genetic engineering a vector must have

1. An origin for replication
2. Low copy number
3. Low rates of multiplication
4. Many cloning sites for the same enzyme

In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was

1. Methylase

2. Exonuclease

3. Aminotransferase

4. Terminal Transferase

 E.coli is  a commonly used host for gene cloning because

1. It is free from elements that interfere with replication and recombination of DNA

2. It is easy to transform

3. It supports replication of inserted DNA.

4. All of these

A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

a.  Suppression vector

b. Expression vector

c. Cloning vector

d. None of these

Electroporation facilitates insertion of foreign DNA into the cell by

1. Changing the porocity of the membrane

2. Changing the electrical potential of the membrane

3. Lysing the cell wall

4. Active transport

The names associated with construction of first Recombinant DNA are

a. Arber, Nathans, Smith

b. Annie Chang, Boyer, Berg, Cohen

c. Howard Temin, Brenner, Sharp

d. Tim Hunt, Hartwell, Nurse

The restriction enzymes most commonly used in rDNA technology are

1. Type 1 Restriction enzymes

2. Type II Restriction enzymes

3. Type III Restriction enzymes

4. Type IV Restriction enzymes

Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.

A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
a. Southern blotting and South Western blotting
b. Northern blotting and western blotting
c. Western blotting and Eastern blotting
d. Southern and Northern blotting

Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants