Q. No.Restriction enzymes produced by some bacteria:
1. cleave phosphodiester bond in the main chromosomal DNA of the bacterium.
2. cut single stranded DNA at random positions.
3. prevent the multiplication of bacteriophage in bacteria.
4. provide resistance against antibiotics.
1. cleave phosphodiester bond in the main chromosomal DNA of the bacterium.
2. cut single stranded DNA at random positions.
3. prevent the multiplication of bacteriophage in bacteria.
4. provide resistance against antibiotics.
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
The DNA fragments separated on agar gel by electrophoresis can be visualised:
1. without staining in the visible light
2. without staining on exposure to UV light
3. after staining with polyethylene glycol followed by exposure to IR radiation
4. after staining with ethidium bromide followed by exposure to UV radiation
1. PstI
2. PvuII
3. BamH I
4. EcoR I
If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
1. 1
2. 2
3. 4
4. 8
A plasmid has a single recognition sequence for EcoRI. When cut with this enzyme, the number of resultant fragments will be:
1. 1
2. 2
3. 3
4. 4
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
a. DNA fragments are negatively charged molecules and can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.
b. The separation of DNA fragments is possible because they differ in size.
c. Smaller the size of the DNA fragment, shorter the distance it travels through agarose gel.
d. Pure DNA can be visualized directly by exposing it to UV radiation without any staining.
Choose the correct answer from the options given below:
1. Only (c) and (d)
2. Only (a) and (c)
3. Only (b) and (c)
4. Only (a) and (b)
| 1. | Bright orange colour | 2. | Bright red colour |
| 3. | Bright Blue colour | 4. | Bright yellow colour |
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