The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

Subtopic:  Polymerase Chain Reaction: PCR |
 94%
From NCERT
NEET - 2016
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The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

Subtopic:  Introduction & History: II | Tools: Enzymes: II | Introduction & History | Tools |
 94%
From NCERT
NEET - 2015
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The DNA molecule to which the gene of interest is integrated for cloning is called:

1. Transformer 2. Vector
3. Template 4. Carrier 
Subtopic:  Tools: Vectors: I | Tools |
 84%
From NCERT
NEET - 2015
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic:  Tools: Vectors: I | Tools |
 75%
NEET - 2014
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

(1) centrifugation

(2) polymerase chain reaction

(3) electrophoresis

(4) restriction mapping

Subtopic:  Process of Biotech |
 88%
From NCERT
NEET - 2013
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Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 93%
From NCERT
NEET - 2013
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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

(1) Non-recombinant bacteria containing β-galactosidase

(2) Insertional inactivation of α-galactosidase in non-recombinant bacteria

(3) Insertional inactivation of β-galactosidase in recombinant bacteria

(4) Inactivation of glycosidase enzyme in recombinant bacteria

 

Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
 67%
From NCERT
NEET - 2013
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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme

2. rop-reduced osmotic pressure

3. Hind III, Eco RI -selectable markers

4. ampR, tetR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
 84%
From NCERT
NEET - 2012
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Which one of the following is a case of wrong matching ?

1. Somatic hybridisation - Fusion of two diverse cells

2. Vector DNA - Site for tRNA synthesis

3. Micropropogation - In vitro production of plants in large numbers

4. Callus - Unorganised mass of cells produced in tissue culture

Subtopic:  Tools: Vectors: I | Tools |
 73%
NEET - 2012
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Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

Subtopic:  Large Scale Production: I | Large Scale Production |
 76%
From NCERT
NEET - 2012
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