Restriction enzymes do not act on the DNA of the Host cell in which they are produced because:

1.	Host DNA is packed into chromosomes
2.	Restriction enzymes are ineffective on host DNA
3.	Host DNA does not have the restriction site for the Restriction enzymes
4.	Restriction site of host DNA is methylated

Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?

In microinjection, the rDNA :

To isolate DNA in pure form from a bacterial cell, it should initially be treated with:

The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase

The downstream processing stage of rDNA does not involve:

A circular plasmid of 10000 bp is digested with two restriction enzymes A and B, to produce 3000 bp and 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A [A1] is present at the 100th base, the order in which the remaining sites [A2, B1 and B2] are present is: 

In-gel electrophoresis, separated DNA fragments can be visualized with the help of:

1. Ethidium bromide in UV radiation

2. Acetocarmine in UV radiation

3. Ethidium bromide in infrared radiation

4. Acetocarmine in bright blue light