Q. No.DNA fragments resulting from restriction enzyme digestion are moved through agar gel during electrophoresis. Which of the following modifications to the fragments will not affect the rate of their movement?
1. Neutralizing the negative charge
2. Increasing length
3. Changing the base composition
4. Decreasing length
1. Neutralizing the negative charge
2. Increasing length
3. Changing the base composition
4. Decreasing length
If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its number:
| 1. | more than the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 2. | equal to the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 3. | equal to the number of copy number of bacteriophages in the cells and less than the copy number of plasmid in the cell. |
| 4. | less than the number of copy number of bacteriophages in the cells and more than the copy number of plasmid in the cell. |
Restriction enzymes do not act on the DNA of the Host cell in which they are produced because:
| 1. | Host DNA is packed into chromosomes |
| 2. | Restriction enzymes are ineffective on host DNA |
| 3. | Host DNA does not have the restriction site for the Restriction enzymes |
| 4. | Restriction site of host DNA is methylated |
1. Agrobacterium tumefaciens
2. Escherichia coli
3. Bacillus thuringiensis
4. Xanthomonas citri
Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
In microinjection, the rDNA :
| 1. | coats the micropellets and is used in biolistics to transform plant cells |
| 2. | is directly injected into the cytoplasm of a plant or an animal cell |
| 3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
| 4. | is directly injected into the nucleus of an animal cell |
To isolate DNA in pure form from a bacterial cell, it should initially be treated with:
| 1. | Proteases and RNase but not DNase | 2. | Lysozyme |
| 3. | Restriction endonuclease | 4. | DNA ligase |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
A circular plasmid of 10000 bp is digested with two restriction enzymes A and B, to produce 3000 bp and 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A [A1] is present at the 100th base, the order in which the remaining sites [A2, B1 and B2] are present is:
| 1. | 5100, 3100, 8100 | 2. | 3100, 5100, 8100 |
| 3. | 8100, 5100, 3100 | 4. | 8100, 3100, 5100 |
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