Q. No.The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI
2. HindII
3. SmaI
4. BamHI
1. EcoRI
The following palindrome is recognized by the restriction enzyme:
1. BamHI
2. EcoRI
3. HindII
4. PstI
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid
2. Bacteriophages
3. BAC
4. Plasmid
The DNA fragments separated on an agarose gel can be visualised after staining with
1. Bromophenol blue
2. Acetocarmine
3. Aniline blue
4. Ethidium bromide
Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'
Restriction endonucleases present in bacterial cells provide an advantage to the cell because these enzymes:
| 1. | can identify the mutated sequences on the chromosomal DNA and help in their repair |
| 2. | selectively bind to particular nucleotide sequences that may appear in viral DNA preventing its replication in the cell |
| 3. | can cut the plasmid DNA of the bacterial cell that allows them to recombine foreign DNA into their plasmid DNA |
| 4. | help the bacterium take up foreign DNA from their environment and combine it into their genome |
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
| 1. | Chilled chloroform | 2. | Isopropanol |
| 3. | Chilled ethanol | 4. | Methanol at room temperature |
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
Biolistic technique is used in:
1. Gene transfer process
2. Tissue culture process
3. Hybridization process
4. Germplasm conservation process
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