Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?
1. BamHI
2. Psti
3. Clal
4. Pvull
Microinjection is suitable for :
1. Injecting an ovum into the sperm in IVF
2. Transforming animal cells
3. Injecting very small sized drug particles into neurons
4. Conferring antibiotic resistance to a certain strain of bacteria
The plasmid pBR322 does not contain:
1. An origin of replication site
2. A gene that encodes for restrictor of plasmid copy number
3. Gene for Ampicillin and Streptomycin resistance
4. Sites for many restriction enzymes
A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of:
1. BamH I and Sma I
2. Hind II and Sma I
3. BamH I and SaI I
4. SaI I and Hind II
The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, Eco RI -selectable markers
4. ampR, tetR-antibiotic resistance genes