Identify a character that is not desirable in a cloning vector:

1. an inactive promoter

2. an origin of replication site

3. selectable markers such as genes for antibiotic resistance

4. one or more unique restriction endonuclease sites

The DNA molecule to which the gene of interest is integrated for cloning is called:

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Which one of the following is a case of wrong matching ?

1. Somatic hybridisation - Fusion of two diverse cells

2. Vector DNA - Site for tRNA synthesis

3. Micropropogation - In vitro production of plants in large numbers

4. Callus - Unorganised mass of cells produced in tissue culture

Which of these are most widely used in genetic engineering?

1. Plastid

2. Plasmid

3. Mitochondrion

4. Ribosome