The first type II restriction endonuclease discovered that could cut dsDNA specific site was:
1. EcoRI
2. SmaI
3. Hind II
4. Hind III
Which statement regarding restriction endonucleases is NOT correct?
1. They recognize a specific base sequence in the DNA.
2. They are produced by bacterial cells as a primitive immune system.
3. They digest DNA by removing nucleotides from a free 3' end.
4. They often generate stick ends.
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
(1) Eco RI
(2) taq polymerase
(3) polymerase III
(4) ligase
Which of the following is a endonuclease?
(1) Protease
(2) DNase I
(3) RNase restriction
(4) Hind II
The cutting of DNA at specific locations became possible with the discovery of
(1) restriction enzymes
(2) probes
(3) selectable markets
(4) ligases