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Q. No.The enzyme DNA ligase, important in rDNA procedures, catalyzes the formation of:
| 1. | N- Glycosidic | 2. | Hydrogen |
| 3. | Phosphodiester | 4. | Ester |
Subtopic: Â General Design of an rDNA experiment |
 64%
Level 2: 60%+
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Histones | 2. | Polysaccharides |
| 3. | RNA | 4. | DNA |
Subtopic: Â Separation and Isolation of DNA fragments |Â General Design of an rDNA experiment |
 89%
Level 1: 80%+
NEET - 2021
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The source of the reverse transcriptase used in recombinant DNA technology, is a:
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
Subtopic: Â General Design of an rDNA experiment |
 84%
Level 1: 80%+
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It is possible to make human proteins in bacterial cells with rDNA experiments because:
| 1. | bacterial cells replicate faster than eukaryotic cells |
| 2. | genetic code is almost universal |
| 3. | bacterial cells have plasmids that can be used as a vector |
| 4. | bacterial cells produce restriction enzymes |
Subtopic: Â General Design of an rDNA experiment |
 53%
Level 3: 35%-60%
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Â Tools |Â General Design of an rDNA experiment |
 75%
Level 2: 60%+
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Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: Â General Design of an rDNA experiment |
 79%
Level 2: 60%+
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The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
Subtopic: Â General Design of an rDNA experiment |
 93%
Level 1: 80%+
NEET - 2022
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During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic: Â General Design of an rDNA experiment |
 88%
Level 1: 80%+
NEET - 2013
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Assertion (A): DNA ligase plays important role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â General Design of an rDNA experiment |
 91%
Level 1: 80%+
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Assertion (A): An alien DNA is linked with the origin of replication for making multiple identical copies.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Â General Design of an rDNA experiment |
 84%
Level 1: 80%+
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