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Q. No.Restriction enzymes are synthesized by:
1. Bacteria only
2. Yeast and bacteria only
3. Eukaryotic cells only
4. All kinds of cells
Subtopic: Â Restriction Enzymes: Historical Background |
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Level 3: 35%-60%
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The thermostable enzymes, Taq and Pfu, Isolated from thermophilic bacteria are:
1. RNA polymerases
2. DNA ligases
3. DNA polymerases
4. Restriction endonucleases
Subtopic: Â Restriction Enzymes: Historical Background |
 77%
Level 2: 60%+
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First discovered restriction endonuclease that always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs is:
1. EcoRI
2. Adenosine deaminase
3. Thermostable DNA polymerase
4. Hind II
Subtopic: Â Restriction Enzymes: Historical Background |
 86%
Level 1: 80%+
NEET - 2020
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For their work in the discovery and characterization of restriction enzymes, the 1978 Nobel Prize for Physiology or Medicine was awarded to:
| 1. | Salvador Luria, Jean Weigle and Giuseppe Bertani |
| 2. | Nirenberg, Matthaei and Korana |
| 3. | Paul Berg, Stanley Cohen and Herbert Boyer |
| 4. | Werner Arber, Daniel Nathans, and Hamilton O. Smith |
Subtopic: Â Restriction Enzymes: Historical Background |
Level 4: Below 35%
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The discipline of biotechnology was founded on the breakthrough work of:
| 1. | Bolivar and Rodriquez | 2. | Cohen and Boyer |
| 3. | Nathans and Daniels | 4. | Redi and Spallanzani |
Subtopic: Â Introduction & History: I |Â Introduction & History: II |Â Restriction Enzymes: Historical Background |
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Which of the following statements will be correct regarding restriction endonucleases used in constructing recombinant DNA molecules?
| 1. | They are RNA molecules acting as enzymes. |
| 2. | The enzymes used in such experiments cut dsDNA randomly at any site. |
| 3. | They have been isolated from bacterial cells. |
| 4. | All restriction enzymes are thermostable and hence can be used in PCR procedures. |
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Restriction Enzymes: Historical Background |
 76%
Level 2: 60%+
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Match column I with column II and choose the correct option:
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
| Column I | Column II | ||
| a. | Salmonella typhimurium | (i) | Source of EcoRI |
| b. | Bacillus thuringiensis | (ii) | Used in developing transgenic tobacco plant |
| c. | Agrobacterium tumefaciens |
(iii) | Used in creating first recombinant DNA |
| d. | Escherichia coli | (iv) | Gene used in creating biopesticide |
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Transforming Plant & Animal Cell |Â Restriction Enzymes: Historical Background |
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Level 1: 80%+
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Restriction enzymes produced by some bacteria:
| 1. | cleave phosphodiester bond in the main chromosomal DNA of the bacterium. |
| 2. | cut single stranded DNA at random positions. |
| 3. | prevent the multiplication of bacteriophage in bacteria. |
| 4. | provide resistance against antibiotics. |
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Restriction Enzymes: Historical Background |
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Level 3: 35%-60%
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Â Restriction Enzymes: Historical Background |Â Tools |
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
| 1. | 6 bp | 2. | 4 bp |
| 3. | 10 bp | 4. | 8 bp |
Subtopic: Â Restriction Enzymes - Main Enzymes |Â Restriction Enzymes: Historical Background |
 83%
Level 1: 80%+
NEET - 2024
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