Q. No.The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, Eco RI -selectable markers
4. ampR, tetR-antibiotic resistance genes
Which of the following options is correct about Agrobacterium tumifaciens?
| 1. | It is a pathogen of angiosperms |
| 2. | It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen |
| 3. | Its Ti plasmid is now modified into an expression vector |
| 4. | Its Ti plasmid is modified into a cloning vector which is no more pathogenic |
pBR322 means
1. BR for the initials of two scientists who constructed it
2. BR for the resistance of BamH1
3. BR for the multiple cloning site
4. BR for its discovery in Berlin
rop segment codes for pBR322?
| 1. | Resistance protein |
| 2. | Protein for replication |
| 3. | Proteins for translation |
| 4. | Cloning site |
BamHI restriction site is present in pBR322 at?
| 1. | rop |
| 2. | Tetracycline resistance gene |
| 3. | Ampicillin resistance gene |
| 4. | Ori |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
A donor DNA and plasmid vector DNA of E. coli are cut by EcoRI. The plasmid contains genes for resistance to ampicillin and tetracycline. The ECoRI recognition sequence lies within the gene for tetracycline resistance. Ligase is used, and the recombinant DNA is produced. The plasmids are transferred to E.coli with the help of electroporation. Samples of the bacterial colonies are then grown in four different media: nutrient medium plus ampicillin, nutrient medium plus tetracycline, nutrient medium plus ampicillin and tetracycline, and nutrient medium without antibiotics. Non-recombinant transformants will grow on:
| 1. | the nutrient medium plus ampicillin, but not on the medium containing tetracycline. |
| 2. | only on the medium containing both antibiotics. |
| 3. | on the medium containing tetracycline, but not on the medium containing ampicillin. |
| 4. | on all four types of mediums. |
A gene of interest is inserted into the lac Z gene in a plasmid also containing a tetracycline-resistant gene. If the transformed bacteria are plated on a medium containing tetracycline and X-gal, the recombinant plasmids will be shown as:
| 1. | a colony which did not grow on the tetracycline plates |
| 2. | a white colony on the tetracycline plates |
| 3. | a blue colony on the tetracycline plates |
| 4. | a black colony on the tetracycline plates |
If we are able to link an alien piece of DNA with bacteriophage or plasmid DNA, we can multiply its number:
| 1. | more than the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 2. | equal to the number of copy number of bacteriophages in the cells and equal to the copy number of plasmid in the cell. |
| 3. | equal to the number of copy number of bacteriophages in the cells and less than the copy number of plasmid in the cell. |
| 4. | less than the number of copy number of bacteriophages in the cells and more than the copy number of plasmid in the cell. |
1. Agrobacterium tumefaciens
2. Escherichia coli
3. Bacillus thuringiensis
4. Xanthomonas citri
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