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Q. No.Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
| 1. | Bright orange colour | 2. | Bright red colour |
| 3. | Bright Blue colour | 4. | Bright yellow colour |
Subtopic: Separation and Isolation of DNA fragments |
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
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In gene gun method used to introduce alien DNA into host cells, microparticles of ___________ metal are used.
| 1. | Silver | 2. | Copper |
| 3. | Zinc | 4. | Tungsten or gold |
Subtopic: Transforming Plant & Animal Cell |
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
Choose the correct answer from the options given below:
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
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Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumifaciens
4. Bacillus thuringiensis
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumifaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322 ?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
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Which of the following statement is incorrect above Agrobacterium tumifaciens ?
| 1. | It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. |
| 2. | 'Ti' plasmid from Agrobacterium tumifaciens used for gene transfer is not pathogenic to plant cells. |
| 3. | It transforms normal plant cells into tumor cells |
| 4. | It delivers 'T-DNA' into plant cell |
Subtopic: Transforming Plant & Animal Cell |
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Match List - I with List - II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | II | III | I | IV |
| 2. | III | I | IV | II |
| 3. | IV | III | I | II |
| 4. | II | IV | I | III |
Subtopic: Cloning Vector |
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Which of the following can act as molecular scissors?
| 1. | Restriction enzymes | 2. | DNA ligase |
| 3. | RNA polymerase | 4. | DNA polymerase |
Subtopic: Restriction Enzymes - Main Enzymes |
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R)
In the light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
| Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is not correct but (R) is correct. |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 4. | (A) is correct but (R) is not correct. |
Subtopic: Selectable Markers: I | Polymerase Chain Reaction: PCR |
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