1. | Enzyme Linked Immuno-Sorbent Assay (ELISA) technique |
2. | Recombinant DNA Technology |
3. | Serum and Urine analysis |
4. | Polymerase Chain Reaction (PCR) technique |
1. | Mature insulin molecule consists of three polypeptide chains - A, B and C. |
2. | Insulin is synthesized as prohormone which contains extra stretch of C-peptide. |
3. | C-peptide is not present in mature insulin molecule. |
4. | Polypeptide chains A and B are linked to disulphide bridges. |
List-I | List-II | ||
(A) | Gene therapy | (I) | Separation of DNA fragments |
(B) | RNA interference | (II) | Diagnostic test for AIDS |
(C) | ELISA | (III) | Cellular defence |
(D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
Options: | (A) | (B) | (C) | (D) |
1. | IV | I | II | III |
2. | IV | II | III | I |
3. | IV | III | II | I |
4. | IV | III | I | II |
ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography
1. | Genetically engineered lymphocytes are not immortal cells. |
2. | Retroviral vector is introduced into these lymphocytes. |
3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
4. | Lymphocytes from patient's blood are grown in culture, outside the body. |
a. | Pro-hormone insulin contain extra stretch of C-peptide |
b. | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
c. | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
d. | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
e. | Some patients develop allergic reactions to the foreign insulin. |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |