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Q. No.Which of the following statements are not true regarding restriction endonucleases?
Choose the answer from the options given below:
| A. | They are called molecular scissors. |
| B. | These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. |
| C. | They cut the DNA only at the centre of the palindromic sites. |
| D. | They remove nucleotides only from the ends of DNA fragments. |
| E. | They recognise specific palindromic base-pair sequences. |
Choose the answer from the options given below:
| 1. | A and B only |
| 2. | D and E only |
| 3. | C and D only |
| 4. | A and E only |
Subtopic: Restriction Enzymes - Main Enzymes |
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Match List-I with List-II:
Choose the correct answer from the options given below :
1. A-II, B-I, C-IV, D-III
2. A-I, B-IV, C-III, D-II
3. A-II, B-III, C-I, D-IV
4. A-I, B-II, C-IV, D-III
| List-I | List-II | ||
| A. | Genetically modified organism | I. | Agrobacterium tumefaciens |
| B. | Thermostable DNA | II. | Bt cotton |
| C. | Ti plasmid | III. | Thermus aquaticus |
| D. | pBR322 | Iv. | Eschericihia coli |
Choose the correct answer from the options given below :
1. A-II, B-I, C-IV, D-III
2. A-I, B-IV, C-III, D-II
3. A-II, B-III, C-I, D-IV
4. A-I, B-II, C-IV, D-III
Subtopic: Process of Biotech |
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Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :
1. Denaturation → Annealing → Extension
2. Denaturation → Extension → Annealing
3. Extension → Annealing → Denaturation
4. Annealing → Denaturation → Extension
1. Denaturation → Annealing → Extension
2. Denaturation → Extension → Annealing
3. Extension → Annealing → Denaturation
4. Annealing → Denaturation → Extension
Subtopic: Polymerase Chain Reaction: PCR |
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Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
| A. | The cutting of DNA is done by molecular scissors. |
| B. | The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. |
| C. | The separated DNA fragments can be seen without staining when exposed to UV light. |
| D. | The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. |
Choose the correct answer from the options given below:
1. A and D only
2. B and D only
3. B and C only
4. A and B only
Subtopic: Separation and Isolation of DNA fragments |
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Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :
| 1. | Gentamycin |
| 2. | Ampicillin and tetracycline |
| 3. | Tetracycline |
| 4. | Ampicillin |
Subtopic: Cloning Vector |
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
In the light of the above statements, choose the most appropriate answer from the options given below:
| Assertion (A): | In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. |
| Reason (R): | Isolation of genetic material needs disruption of cells. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | (A) is not correct but (R) is correct |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A) |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A) |
| 4. | (A) is correct but (R) is not correct |
Subtopic: Separation and Isolation of DNA fragments |
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Match List-I with List-II:
Choose the correct answer from the options given below :
1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I
| List-I | List-II | ||
| A. | Transformation | I. | Restriction enzyme |
| B. | Cloning site | II. | Transfer DNA to host bacteria |
| C. | Selection | III. | Replication |
| D. | Ori | IV. | Antibiotic |
Choose the correct answer from the options given below :
1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I
Subtopic: Cloning Vector |
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Given below are two statements:
In the light of the above statements, choose the most appropriate answer from the options given below:
| Statement I: | Plasmids are autonomously replicating DNA. |
| Statement II: | Plasmids are extrachromosomal DNA. |
In the light of the above statements, choose the most appropriate answer from the options given below:
| 1. | Statement I is incorrect but Statement II is correct |
| 2. | Both Statement I and Statement II are correct |
| 3. | Both Statement I and Statement II are incorrect |
| 4. | Statement I is correct but Statement II is incorrect |
Subtopic: Cloning Vector | Principles of Biotechnology |
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During PCR, primers bind to the DNA strands in the __________ step.
1. ligation
2. denaturation
3. extension
4. annealing
1. ligation
2. denaturation
3. extension
4. annealing
Subtopic: Polymerase Chain Reaction: PCR |
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In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
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