Q. No.What would be true for both Eukaryotic and Prokaryotic gene expression?
| 1. | Coupling of transcription and translation |
| 2. | Post-transcriptional removal of introns |
| 3. | Binding of RNA polymerase to the promoter to initiate transcription |
| 4. | The eukaryotic hnRNA and prokaryotic RNA are polycistronic |
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Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?
| 1. | They only increase the expression |
| 2. | They only decrease the expression |
| 3. | They interact with RNA polymerase but do not affect the expression |
| 4. | They can act both as activators and as repressors |
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What is it that forms the basis of DNA Fingerprinting?
| 1. | The relative proportions of purines and pyrimidines in DNA. |
| 2. | The relative difference in the DNA occurrence in blood, skin, and saliva. |
| 3. | The relative amount of DNA in the ridges and grooves of the fingerprints. |
| 4. | Satellite DNA occurring as highly repeated short DNA segments |
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DNA fingerprinting is related to:
| 1. | Molecular analysis of profiles of DNA samples |
| 2. | Analysis of DNA samples using imprinting devices |
| 3. | Techniques used for molecular analysis of different specimens of DNA |
| 4. | Techniques used in the identification of fingerprints of different persons |
What would be the actual inducer for the lac operon?
| 1. | lactose | 2. | allolactose |
| 3. | ß-galactosidase | 4. | galactose |
The primary RNA transcript in eukaryotes contains introns. This can be demonstrated by:
| I: | A comparison of the DNA and mRNA sequences. |
| II: | A comparison of the genomic DNA and cDNA sequences. |
| III: | A hybridization between DNA and mRNA molecules. |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Discontinuous synthesis of DNA occurs in one strand, because:
| 1. | DNA molecule being synthesised is very long |
| 2. | DNA-dependent DNA polymerase catalyses polymerisation only in one direction (5’ → 3’) |
| 3. | it is a more efficient process |
| 4. | DNA ligase joins the short stretches of DNA |
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Match each item in Column I with one item in Column II and chose your answer from the codes given below:
|
Column I (Scientist) |
Column II (Contribution) |
|
I. Hargobind Khorana II. Norman Borlaug III. Cohen and Boyer IV. Ian Wilmut |
1. Green revolution 2. First man-made gene 3. First cloned mammal 4. rDNA technology |
| Codes: | I | II | III | IV |
| 1. | 1 | 2 | 3 | 4 |
| 2. | 2 | 1 | 4 | 3 |
| 3. | 2 | 1 | 3 | 4 |
| 4. | 1 | 2 | 4 | 3 |
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Using reverse transcriptase or gene synthesis has an advantage over the shotgun approach in obtaining a copy of the gene required as:
| 1. | the gene produced can easily be ligated to the vector |
| 2. | the gene produced is not a split gene |
| 3. | the gene produced does not require a promoter to be expressed |
| 4. | the gene produced does not require an ori to replicate |
Heat-killed S form Streptococcus extract treated with proteases is mixed with live R form Streptococcus cells and injected into living mice. What is most likely to happen?
| 1. | The mice would live. |
| 2. | The mice would die. |
| 3. | The mice's blood would have dead R cells. |
| 4. | A new strain of Streptococcus will be present in mice. |
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