Q. No.A cis acting factor that place very important role in eukaryotic transcription will be:
1. RNA polymerase II.
2. Transcription factors.
3. Enhancers.
4. Shine Dalgarno sequence
Name the enzyme that facilitates opening of DNA helix during transcription.
1. DNA helicase
2. DNA polymerase
3. RNA polymerase
4. DNA ligase
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A ribosomal binding site in bacterial and archaeal messenger RNA, generally located around 8 bases upstream of the start codon AUG is called as:
1. Shine-Dalgarno sequence.
2. Kozak consensus sequence.
3. Sorting signals.
4. Prinbow box.
Let us presume that it is possible to view enzymes and organelles in a cell, including those involved with gene expression. A prokaryotic cell can be differentiated from a eukaryotic cell because it can be seen that:
1. the nucleotides in the promoter are different in prokaryotes and eukaryotes.
2. the actual genes are more widely-spaced apart in eukaryotes than prokaryotes.
3. ribosomes have started translating mRNA that was still being synthesized by RNA polymerase.
4. prokaryotic mRNA does not contain introns.
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The 5’ cap in the final transcript in eukaryotic mRNA helps in:
1. Protection from inappropriate splicing.
2. Synthesis of the polyA tail.
3. Transport of mRNAs into the nucleus.
4. Recognition of the 5' end of the transcript by a ribosome.
Read the following four statements (A-D):
| I. | In transcription, adenosine pairs with uracil |
| II. | Regulation of lac operon by a repressor is referred to as positive regulation |
| III. | The human genome has approximately 50,000 genes |
| V. | Haemophilia is a sex-linked recessive disease |
How many of the above statements are right?
1. Three
2. Four
3. One
4. Two
Hybridization of mRNA with DNA can reveal which segment of the DNA are introns and which segment are exons. When the magnification is high enough to see the molecule, the introns appear as
1. Single stranded loops in a double stranded molecule
2. Separate fragments
3. Triple stranded nucleic acid molecules
4. Barr bodies
In eukaryotes, RNA polymerase II transcribes:
1. heterogenous nuclear RNA
2. small nuclear RNA
3. transfer RNA
4. ribosomal RNA
The predominant site for control of gene expression in prokaryotes is:
1. Transcription initiation
2. Binding of mRNA to ribosome
3. Translation termination
4. Post translational modification
What is the role of RNA polymerase III in the process of transcription in eukaryotes?
1. Transcribes precursor of mRNA
2. Transcribes only snRNAs
3. Transcribes rRNAs (28S, 18S and 5.8S)
4. Transcribes tRNA, 5s rRNA and snRNA
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