Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?
1. restriction endonuclease - production of DNA fragments for gene cloning.
2. DNA polymerase - copies DNA sequences to create primers in the polymerase chain reaction.
3. reverse transcriptase - production of cDNA from mRNA.
4. electrophoresis - RLFP analysis.

During translation, formation of peptide bonds in bacteria is catalyzed by:
1. Polynucleotide phosphorylase
2. Protease
3. Ligase
4. Ribozyme

The enzyme aminoacyl-tRNAsynthetase is able to recognize all the following in the tRNA molecule except:
1. The acceptor stem.
2. Bases in the stem loop regions.
3. The anticodon region.
4. 5’ end.

A sequential expression of a set of human genes occurs when a steroid molecule binds to
1. mRNA
2. DNA sequence
3. tRNA
4. Ribosome

All the following regarding RNA splicing are correct except:
1. Some introns can splice themselves out.
2. Splicing is required for some tRNAs.
3. The final step of splicing is formation of a phosphodiester linkage.
4. Introns often have a specific function after they are removed.

On which organism Beadle and Tatum worked to propose one gene-one enzyme hypothesis
1. Drosophila
2. Escherichia coli
3. Neurospora crassa
4. Nostoc

The first formed primary structure of a protein molecule represents:
1. Folding of protein due to hydrophobic interactions.
2. Local folding which is stabilized by hydrogen bonds.
3. The sequence of amino acids in the polypeptide.
4. Interactions between two or more polypeptides.

To initiate translation, the mRNA first binds to:

1. The smaller ribosomal sub-unit,

2. The larger ribosomal sub-unit

3. The whole ribosome

4. No such specificity exists.