Given below is a schematic metabolic pathway. In an experiment, the concentration of enzyme 1 was increased but the concentration of enzymes 2 and 3 were kept constant. What would happen to the rate of production of D?

1. it would go down
2. it would go up
3. it would stay the same
4. it would stop

Consider the given two statements:
I. The rate determining step for an enzyme-substrate reaction is always the second step in which [ES] is converted into the product.
II. Once the enzyme performs its duty, it is free to do more work.

1. Both I and II are correct and II explains I
2. Both I and are correct but II does not explain I
3. I is correct and II is incorrect
4. Both I and II are incorrect

An enzyme catalyzed reaction has a △G of -30 Kcal/mol. The amount of enzyme is doubled. What will be the △G for the new reaction?
1. -60 kcal/mol
2. -30 kcal/mol
3. +30 kcal/mol
4. +60 kcal/mol