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Q. No.Melting of DNA at 94°C is due to breakdown of
1. Glycosidic bonds
2. Phosphodiester bonds
3. Disulphide bonds
4. Hydrogen bonds
Subtopic: Polymerase Chain Reaction: PCR |
57%
Level 3: 35%-60%
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Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I
| 1. | \(5'C-T-G~\\ 3'-G-A-C-G-T'\) | \(C-A-G3'\\ C'5\) |
| 2. | \(5'C-T\\ 3'G-A-G-C \) | \(G-C-A-G3'\\ T-C'5\) |
| 3. | \(5'C-T-G-C \\ 3'G-A-C-G \) | \(A-G3'\\ T'-C5'\) |
| 4. | \(5'C-T-G-C-A\\ 3'G\) | \(3'G\\ A-C-G-T-C'5\) |
Subtopic: Restriction Enzymes - Main Enzymes |
56%
Level 3: 35%-60%
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Assertion(A): Synthetic oligonucleotide polymers are used during Annealing in a PCR
Reason(R): The primers bind to the double stranded DNA at their complementary regions
Reason(R): The primers bind to the double stranded DNA at their complementary regions
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are true, but (R) is not the correct explanation of (A) |
| 3. | (A) is true, but (R) is false |
| 4. | (A) is false, but (R) is true |
Subtopic: Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
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Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
1. i-S, ii-R, iii-Q
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
| Column I (PCR steps) |
Column II (Temperature) |
||
| i. | Polymerization | P. | \(40-60 ^\circ C\) |
| ii. | Denaturation | Q. | \(70-75^ \circ C\) |
| iii. | Primer annealing | R. | \(90-98 ^ \circ C \) |
| S. | \(28-37^ \circ C\) |
2. i-R, ii-S, iii-P
3. i-Q, ii-R, iii-P
4. i-P, ii-R, iii-S
Subtopic: Polymerase Chain Reaction: PCR |
63%
Level 2: 60%+
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From the following identify the INCORRECT pair.
| Restriction enzyme | Source | |
| 1. | AluI | Arthrobacter luteus |
| 2. | EcoRI | Esherichia coli Ry13 |
| 3. | BamHI | Bacillus thuringiensis H |
| 4. | HindII | H. influenza Rd |
Subtopic: Tools: Vectors: I |
62%
Level 2: 60%+
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From the following identify the INCORRECT pair.
| Restriction enzyme | Type of ends produced | |
| 1. | AluI | Blunt ends |
| 2. | EcoRI | Sticky ends |
| 3. | BamHI | Sticky ends |
| 4. | HindII | Sticky ends |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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Match the following enzymes with their functions:
Select the correct option from the following:
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
| Column I | Column II | ||
| i. | Restriction endonuclease | a. | Joins the DNA fragments |
| ii. | Restriction exonucleases | b. | Extends primers on genomic DNA template |
| iii. | DNA ligase | c. | Cuts DNA at Specific position |
| iv. | Taq polymerase | d. | Removes nucleotides from the ends of DNA |
1. i-b, ii-d, iii-a, iv-c
2. i-c, ii-a, iii-d, iv-b
3. i-c, ii-d, iii-a, iv-b
4. i-d, ii-c, iii-a, iv-b
Subtopic: Restriction Enzymes - Main Enzymes |
84%
Level 1: 80%+
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Study the following lists:
1. (i-c); (ii-e); (iii-d); (iv-b)
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
| List I | List II | ||
| i. | Vector | a. | Resistant to cotton bollworms. |
| ii. | Downstream processing | b. | Mobile genetic elements |
| iii. | Cry II AB | c. | Controls corn borer |
| iv. | Transposons | d. | Ti plasmid |
| e. | Purifying protein in biopharmaceuticals. |
2. (i-d); (ii-e); (iii-b); (iv-c)
3. (i-d); (ii-e); (iii-a); (iv-b)
4. (i-d); (ii-b); (iii-a); (iv-e)
Subtopic: Bt Crops |
83%
Level 1: 80%+
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Match the following.
The CORRECT answer is
| List I | List II | List II | |||
| i. | Hind III | a. | Agarose gel | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marker |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequences | 4. | Transgenic plant |
| i | ii | iii | iv | |
| 1. | d,1 | a,3 | c,3 | b,4 |
| 2. | d,1 | c,2 | a,3 | b,4 |
| 3. | d,1 | c,2 | b,4 | a,3 |
| 4. | a,3 | b,4 | c,2 | d,1 |
Subtopic: Tools: Vectors: I |
76%
Level 2: 60%+
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Study the following lists
| List I | List II | ||
| 1 | Eco RI | I | Vector for transgenic plants |
| 2 | Ti plasmid | II | Restriction enzyme |
| 3 | pBR 322 | III | Probe |
| 4 | Reverse transcriptase | IV | RNA dependent DNA synthesis |
| V | Artificial plasmid |
| 1 | 2 | 3 | 4 | |
| 1. | II | I | V | IV |
| 2. | I | II | III | IV |
| 3. | II | I | IV | V |
| 4. | II | I | IV | III |
Subtopic: Tools: Vectors: I |
73%
Level 2: 60%+
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