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Q. No.Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
90%
Level 1: 80%+
NEET - 2023
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
85%
Level 1: 80%+
NEET - 2023
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Which of the following statement is incorrect about Agrobacterium tumefaciens ?
| 1. | It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. |
| 2. | 'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells. |
| 3. | It transforms normal plant cells into tumor cells. |
| 4.. | It delivers 'T-DNA' into plant cell. |
Subtopic: Transforming Plant & Animal Cell |
61%
Level 2: 60%+
NEET - 2023
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
55%
Level 3: 35%-60%
NEET - 2023
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Which of the following can act as molecular scissors?
1. Restriction enzymes
2. DNA ligase
3. RNA polymerase
4. DNA polymerase
1. Restriction enzymes
2. DNA ligase
3. RNA polymerase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
94%
Level 1: 80%+
NEET - 2023
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Genetic variation cannot be effectively brought about by?
1. Transduction
2. Mutation
3. Transformation
4. Amplification
1. Transduction
2. Mutation
3. Transformation
4. Amplification
Subtopic: Polymerase Chain Reaction: PCR |
66%
Level 2: 60%+
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Which is also called molecular glue?
1. DNA gyrase
2. DNA helicase
3. DNA ligase
4. DNA polymerase
1. DNA gyrase
2. DNA helicase
3. DNA ligase
4. DNA polymerase
Subtopic: Tools |
95%
Level 1: 80%+
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In annealing:
| 1. | Primers anneal to the DNA template |
| 2. | Taq polymerase adds nucleotides to the annealed primer |
| 3. | Two strands of the DNA separate |
| 4. | Temperature in 92° C |
Subtopic: Polymerase Chain Reaction: PCR |
63%
Level 2: 60%+
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In Annealing:
| 1. | Primers anneal to the DNA template |
| 2. | Taq polymerase adds nucleotides to the annealed primer |
| 3. | Two strands of the DNA separate |
| 4. | Temperature is 92ºC |
Subtopic: Polymerase Chain Reaction: PCR |
85%
Level 1: 80%+
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Subtopic: Polymerase Chain Reaction: PCR |
94%
Level 1: 80%+
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